Fecha/hora: 03/06/2024 06:55:53

Biblioteca(s): 	INIA Treinta y Tres.	
Fecha corriente: 	03/12/2020	
Fecha de la última actualización: 	03/12/2020	
Autoria: 	LLANES ALVAREZ, Y.; PEÑA BÁRZAGA, I.; BATISTA-LE RIVEREND, L.; PACHECO, R.; ZAMORA RODRÍGUEZ, V.; BENÍTEZ-GALEANO, M.J.; RIVAS, F.; BERTALMIO, A.; HERNÁNDEZ RODRÍGUEZ, L.	
Afiliación: 	YILIAN LLANES-ALVAREZ, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba.; INÉS PEÑA-BÁRZAGA, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba.; LOCHY BATISTA-LE RIVEREND, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba.; RONAL PACHECO, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba. Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, México.; VICTORIA ZAMORA-RODRÍGUEZ, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba.; MARÍA JOSÉ BENÍTEZ-GALEANO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. Laboratorio de Virología Molecular, Universidad de La República, Uruguay.; CARLOS FERNANDO RIVAS GRELA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA MARIA BERTALMIO CASARIEGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LESTER HERNÁNDEZ RODRÍGUEZ, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba. INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.	
Título: 	Prevalence of mild citrus tristeza virus isolates of the T30 genotype in Cuban commercial citrus fields after the dissemination of huanglongbing.	
Año de publicación: 	2020	
Fuente/Imprenta: 	Crop Protection, 2021, v. 140, art. 105422. DOI: https://doi.org/10.1016/j.cropro.2020.105422	
Páginas: 	11 p.	
DOI: 	10.1016/j.cropro.2020.105422	
Idioma: 	Inglés	
Notas: 	Article history: Received 5 May 2020; Received in revised form 8 October 2020; Accepted 12 October 2020; Available online 23 October 2020.	
Contenido: 	Huanglongbing (HLB) disease detection in Cuba in 2006 led to a turn in citrus management in the country, including the production of certified budwood in protected nurseries, eradication of symptomatic plants and old orchards, and chemical control of the vector Diaphorina citri. This dramatic shift in citrus farming could have affected the incidence and composition of citrus tristeza virus (CTV) populations. This work aimed to determine CTV incidence and characterize viral isolates from the main Cuban citrus-producing areas. A survey throughout the country was carried out which resulted in the collection of 27 CTV isolates. Results showed a decrease of CTV incidence in western and central areas of the country, but an increase in the East, compared to previous reports. Bioindexing showed that most of the isolates induced none or mild symptoms. Serological characterization using seven different CTV specific monoclonal antibodies (MAb) showed heterogeneity among the 27 isolates. Six out of twenty-seven were MCA13-reactive, a MAb associated to the presence of severe CTV isolates. Nevertheless, sequence comparison of the p20, p23 and p25 genes evidenced a high nucleotide sequence identity among isolates, and according to the phylogenetic analyses, all the sequences belonged to the CTV T30 genotype. Thus, our results showed a decrease in the CTV incidence in the surveyed areas and the broad dissemination of mild CTV isolates of the T30 genotype, which could be partially explained by the shift in the management programs implemented for CTV and HLB control in Cuba.	
Palabras-Clave: 	BIOLOGICAL CHARACTERIZATION; CLOSTEROVIRIDAE; CTV; GENOTYPING; MCA13; SEROLOGICAL CHARACTERIZATION.	
Categoría del tema: 	H20 Enfermedades de las plantas	
Registro original: 	INIA Treinta y Tres (TT)	
Ejemplares: 	TT - (103155-1) - PXI - AP - DD - PP/CROP PROTECTION/2021-Llanes -  -  - DIS